| Class | Compound Name (in order of elution) | Column and Dimensions | Conditions | Chromatogram |
|---|---|---|---|---|
| Beta-Blockers | 1. Alprenolol 2. Labetalol 3. Pindolol 4. Acebutolol 5. Sotalol 6. Metaproteranol 7. Nadolol | FructoShell-N 10x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
| Beta-Blockers | 1. Alprenolol 2. Labetalol 3. Pindolol 4. Acebutolol 5. Sotalol 6. Metaproteranol 7. Nadolol | Frulic-N 15x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
| Beta-Blockers | SilicaShell 10x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
|
| Nucleic Acids, Bases, and Nucleotide | 1. Thymine 2. Uracil 3. Thymidine 4. Uridine 5. 5-fluorocytosine 6. Adenine 7. Cytosine 8. Cytidine 9. Guanosine | FructoShell-N 10x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 90/10 (v/v) Flow rate: 1.0 mL/min UV detection at 254 nm |  |
| Nucleic Acids, Bases, and Nucleotide | 1. Thymine 2. Uracil 3. Thymidine 4. Uridine 5. 5-fluorocytosine 6. Adenine 7. Cytosine 8. Cytidine 9. Guanosine | Frulic-N 15x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 90/10 (v/v) Flow rate: 1.0 mL/min UV detection at 254 nm |  |
| Salicylic Acids | 1. Salicylamide 2. Salicylic acid 3. 3,4-dihydroxyphenylacetic acid 4. Acetylsalicylic acid | FructoShell-N 10x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
| Salicylic Acids | 1. Salicylamide 2. Salicylic acid 3. 3,4-dihydroxyphenylacetic acid 4. Acetylsalicylic acid | Frulic-N 15x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
| Salicylic Acids | 1. Salicylamide 2. Salicylic acid 3. 3,4-dihydroxyphenylacetic acid 4. Acetylsalicylic acid | SilicaShell 10x0.46 cm | Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v) Flow rate: 1.0 mL/min UV detection at 220 nm |  |
| Inorganic Ions | Calcium chloride Lithium bromide Magnesium chloride Potassium bromate Potassium iodide Potassium phosphate Sodium bromide Sodium chlorate | FructoShell-N 10x0.21 cm | Eluent: (A) 100 mM pH 3 Ammonium Formate, (B) Acetonitrile, hold 91% (B) for 1 min, 91-60% (B) in 10 min, 3 min re-equilibration Flow rate: 0.4 mL/min Temperature: 30 °C |  |
| Inorganic Ions | Calcium chloride Lithium bromide Magnesium chloride Potassium bromate Potassium iodide Potassium phosphate Sodium bromide Sodium chlorate | SilicaShell 10x0.21 cm | Eluent: (A) 100 mM pH 3 Ammonium Formate, (B) Acetonitrile, hold 91% (B) for 1 min, 91-60% (B) in 10 min, 3 min re-equilibration Flow rate: 0.4 mL/min Temperature: 30 °C |  |
| Water Soluble Vitamins | (B1) Thiamine (B2) Riboflavin (B3) Niacin/Nicotinic acid (B6) Pyridoxine/Pyridoxol (B9) Folate/Folic acid (B10) 4-aminobenzoic acid/PABA (B12) Cyanocobalamin (C) Ascorbic acid | FructoShell-N 10x0.21 cm | Eluent: (A) 100 mM pH 4.6 Ammonium Acetate with 0.5% Acetic Acid in Water, (B) Acetonitrile Flow rate: 0.5 mL/min, 87% B for 1 min, 87-50% B in 4 min, 3 min re-equilibration Temperature 40 °C UV detection at 260 nm |  |
| Water Soluble Vitamins | (B1) Thiamine (B2) Riboflavin (B3) Niacin/Nicotinic acid (B6) Pyridoxine/Pyridoxol (B9) Folate/Folic acid (B10) 4-aminobenzoic acid/PABA (B12) Cyanocobalamin (C) Ascorbic acid | SilicaShell 10x0.21 cm | Eluent: (A) 100 mM pH 4.6 Ammonium Acetate with 0.5% Acetic Acid in Water, (B) Acetonitrile Flow rate: 0.5 mL/min, 87% B for 1 min, 87-50% B in 4 min, 3 min re-equilibration Temperature 40 °C UV detection at 260 nm |  |