Arlington, TX 1-817-272-1399
HILIC Separations



ClassCompound Name
(in order of elution)
Column and DimensionsConditionsChromatogram
Beta-Blockers1. Alprenolol
2. Labetalol
3. Pindolol
4. Acebutolol
5. Sotalol
6. Metaproteranol
7. Nadolol
FructoShell-N
10x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Beta-Blockers1. Alprenolol
2. Labetalol
3. Pindolol
4. Acebutolol
5. Sotalol
6. Metaproteranol
7. Nadolol
Frulic-N
15x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Beta-BlockersSilicaShell
10x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Nucleic Acids, Bases, and Nucleotide1. Thymine
2. Uracil
3. Thymidine
4. Uridine
5. 5-fluorocytosine
6. Adenine
7. Cytosine
8. Cytidine
9. Guanosine
FructoShell-N
10x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 90/10 (v/v)
Flow rate: 1.0 mL/min
UV detection at 254 nm
Nucleic Acids, Bases, and Nucleotide1. Thymine
2. Uracil
3. Thymidine
4. Uridine
5. 5-fluorocytosine
6. Adenine
7. Cytosine
8. Cytidine
9. Guanosine
Frulic-N
15x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 90/10 (v/v)
Flow rate: 1.0 mL/min
UV detection at 254 nm
Salicylic Acids1. Salicylamide
2. Salicylic acid
3. 3,4-dihydroxyphenylacetic acid
4. Acetylsalicylic acid
FructoShell-N
10x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Salicylic Acids1. Salicylamide
2. Salicylic acid
3. 3,4-dihydroxyphenylacetic acid
4. Acetylsalicylic acid
Frulic-N
15x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Salicylic Acids1. Salicylamide
2. Salicylic acid
3. 3,4-dihydroxyphenylacetic acid
4. Acetylsalicylic acid
SilicaShell
10x0.46 cm
Eluent: ACN/20 mM ammonium acetate, pH 4.0, 85/15 (v/v)
Flow rate: 1.0 mL/min
UV detection at 220 nm
Inorganic IonsCalcium chloride
Lithium bromide
Magnesium chloride
Potassium bromate
Potassium iodide
Potassium phosphate
Sodium bromide
Sodium chlorate
FructoShell-N
10x0.21 cm
Eluent: (A) 100 mM pH 3 Ammonium Formate, (B) Acetonitrile, hold
91% (B) for 1 min, 91-60% (B) in 10 min, 3 min re-equilibration
Flow rate: 0.4 mL/min
Temperature: 30 °C
Inorganic IonsCalcium chloride
Lithium bromide
Magnesium chloride
Potassium bromate
Potassium iodide
Potassium phosphate
Sodium bromide
Sodium chlorate
SilicaShell
10x0.21 cm
Eluent: (A) 100 mM pH 3 Ammonium Formate, (B) Acetonitrile, hold
91% (B) for 1 min, 91-60% (B) in 10 min, 3 min re-equilibration
Flow rate: 0.4 mL/min
Temperature: 30 °C
Water Soluble Vitamins(B1) Thiamine
(B2) Riboflavin
(B3) Niacin/Nicotinic acid
(B6) Pyridoxine/Pyridoxol
(B9) Folate/Folic acid
(B10) 4-aminobenzoic acid/PABA
(B12) Cyanocobalamin
(C) Ascorbic acid
FructoShell-N
10x0.21 cm
Eluent: (A) 100 mM pH 4.6 Ammonium Acetate with 0.5% Acetic Acid in Water, (B) Acetonitrile
Flow rate: 0.5 mL/min, 87% B for 1 min, 87-50% B in 4 min, 3 min re-equilibration
Temperature 40 °C
UV detection at 260 nm
Water Soluble Vitamins(B1) Thiamine
(B2) Riboflavin
(B3) Niacin/Nicotinic acid
(B6) Pyridoxine/Pyridoxol
(B9) Folate/Folic acid
(B10) 4-aminobenzoic acid/PABA
(B12) Cyanocobalamin
(C) Ascorbic acid
SilicaShell
10x0.21 cm
Eluent: (A) 100 mM pH 4.6 Ammonium Acetate with 0.5% Acetic Acid in Water, (B) Acetonitrile
Flow rate: 0.5 mL/min, 87% B for 1 min, 87-50% B in 4 min, 3 min re-equilibration
Temperature 40 °C
UV detection at 260 nm